Antifungal Agent 4-AN Changes the Genome-Wide Expression Profile, Downregulates Virulence-Associated Genes and Induces Necrosis in Candida albicans Cells

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dc.contributor.authorMartyna, Aleksandra
dc.contributor.authorMasłyk, Maciej
dc.contributor.authorJaneczko, Monika
dc.contributor.authorKochanowicz, Elżbieta
dc.contributor.authorGielniewski, Bartłomiej
dc.contributor.authorŚwiercz, Aleksandra
dc.contributor.authorDemchuk, Oleg M.
dc.contributor.authorKubiński, Konrad
dc.date.accessioned2022-06-23T09:49:35Z
dc.date.available2022-06-23T09:49:35Z
dc.date.issued2020
dc.description.abstractIn the light of the increasing occurrence of antifungal resistance, there is an urgent need to search for new therapeutic strategies to overcome this phenomenon. One of the applied approaches is the synthesis of small-molecule compounds showing antifungal properties. Here we present a continuation of the research on the recently discovered anti-Candida albicans agent 4-AN. Using next generation sequencing and transcriptional analysis, we revealed that the treatment of C. albicans with 4-AN can change the expression profile of a large number of genes. The highest upregulation was observed in the case of genes involved in cell stress, while the highest downregulation was shown for genes coding sugar transporters. Real-time PCR analysis revealed 4-AN mediated reduction of the relative expression of genes engaged in fungal virulence (ALS1, ALS3, BCR1, CPH1, ECE1, EFG1, HWP1, HYR1 and SAP1). The determination of the fractional inhibitory concentration index (FICI) showed that the combination of 4-AN with amphotericin B is synergistic. Finally, flow cytometry analysis revealed that the compound induces mainly necrosis in C. albicans cells.pl
dc.identifier.citation"Molecules", 2020, Vol. 25, nr 24, 2928pl
dc.identifier.doi10.3390/molecules25122928
dc.identifier.urihttp://hdl.handle.net/20.500.12153/3230
dc.language.isoenpl
dc.publisherMDPIpl
dc.rightsUznanie autorstwa-Użycie niekomercyjne-Bez utworów zależnych 3.0 Polska*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/pl/*
dc.subject4-ANpl
dc.subjectC. albicanspl
dc.subjectgene expressionpl
dc.subjectsynergypl
dc.subjectantifungalspl
dc.subjectnecrosispl
dc.titleAntifungal Agent 4-AN Changes the Genome-Wide Expression Profile, Downregulates Virulence-Associated Genes and Induces Necrosis in Candida albicans Cellspl
dc.typeinfo:eu-repo/semantics/articlepl
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