Statistical evaluation of growth parameters in biofuel waste as a culture medium for improved production of single cell protein and amino acids by Yarrowia lipolytica

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dc.contributor.authorJach, Monika Elżbieta
dc.contributor.authorBaj, Tomasz
dc.contributor.authorJuda, Marek
dc.contributor.authorŚwider, Robert
dc.contributor.authorMickowska, Barbara
dc.contributor.authorMalm, Anna
dc.date.accessioned2022-09-15T09:32:31Z
dc.date.available2022-09-15T09:32:31Z
dc.date.issued2020
dc.description.abstractYarrowia lipolytica is an oleaginous yeast species with the ability to grow on a number of substrates types, especially industrial wastes. This paper concerns the statistical optimization of fermentation parameters and media to ensure consistent and improved Y. lipolytica protein production. A strain of Y. lipolytica A-101 was observed to be proficient in producing single cell protein, amino acids, and vitamin B12 while utilizing biofuel waste instead of a complete YPD medium for yeast growth. A fractional fractal design experiment was then applied, and the two fermentation param- eters of temperature and pH were recognized to have a significant effect on the protein and amino acid production. Subsequently, the response surface methodology with a three-level complete factorial design was employed to optimize these influential parameters. Therefore, five different measuring systems were utilized to construct a quad- ratic model and a second-order polynomial equation. Optimal levels of parameters were then obtained by analysis of the model and the numerical optimization method. When the Y. lipolytica A-101 was cultivated at optimized pH (5.0) using biofuel waste as a medium, the protein concentration was increased to 8.28—a 44% enhancement as com- pared to the original (3.65). This study has thus demonstrated a beneficial way to cultivate Y. lipolytica A-101 on biofuel waste for enhanced production of single cell protein and amino acids for use in human diet and in animal feed.pl
dc.identifier.citation"AMB Express", 2020, Vol. 10, Article number: 35pl
dc.identifier.doi10.1186/s13568-020-00968-x
dc.identifier.issn2191-0855
dc.identifier.urihttp://hdl.handle.net/20.500.12153/3560
dc.language.isoenpl
dc.publisherSpringerpl
dc.rightsUznanie autorstwa-Użycie niekomercyjne-Bez utworów zależnych 3.0 Polska*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/pl/*
dc.subjectAmino acidspl
dc.subjectBiofuel wastepl
dc.subjectFermentation optimizationpl
dc.subjectSingle cell proteinpl
dc.subjectYarrowia lipolyticapl
dc.titleStatistical evaluation of growth parameters in biofuel waste as a culture medium for improved production of single cell protein and amino acids by Yarrowia lipolyticapl
dc.title.alternativeStatystyczna ocena parametrów wzrostu w odpadach biopaliwowych jako pożywce hodowlanej w celu poprawy produkcji białka i aminokwasów przez Yarrowia lipolyticapl
dc.typeinfo:eu-repo/semantics/articlepl
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