Screening and molecular identification of bacteria from the midgut of Amphimallon solstitiale larvae exhibiting antagonistic activity against bacterial symbionts of entomopathogenic nematodes

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dc.contributor.authorSkowronek, Marcin
dc.contributor.authorSajnaga, Ewa
dc.contributor.authorKazimierczak, Waldemar
dc.contributor.authorLis, Magdalena
dc.contributor.authorWiater, Adrian
dc.date.accessioned2022-03-21T08:12:52Z
dc.date.available2022-03-21T08:12:52Z
dc.date.issued2021-11-05
dc.description.abstractEntomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) are a group of organisms capable of infecting larvae of insects living in soil, including representatives of the family Scarabaeidae. Their insecticidal activity is related to the presence of symbiotic bacteria Xenorhabdus spp. or Photorhabdus spp. in the alimentary tract, which are released into the insect body, leading to its death caused by bacterial toxins and septicemia. Although the antibacterial activities of symbionts of entomopathogenic nematodes have been well described, there is insufficient knowledge of the interactions between these bacteria and microorganisms that naturally inhabit the alimentary tract of insects infested by nematodes. In this study, 900 bacterial strains isolated from midgut samples of Amphimallon solstitiale larvae were tested for their antagonistic activity against the selected five Xenorhabdus and Photorhabdus species. Cross-streak tests showed significant antibacterial activity of 20 isolates. These bacteria were identified as Bacillus [Brevibacterium] frigoritolerans, Bacillus toyonensis, Bacillus wiedmannii, Chryseobacterium lathyri, Chryseobacterium sp., Citrobacter murliniae, Enterococcus malodoratus, Paenibacillus sp., Serratia marcescens and Serratia sp. Since some representatives of the intestinal microbiota of A. solstitiale are able to inhibit the growth of Xenorhabdus and Photorhrhabdus bacteria in vitro, it can be assumed that this type of bacterial interaction may occur at certain stages of insect infection by Steinernema or Heterorhabditis nematodes.pl
dc.description.sponsorshipNCNpl
dc.identifier.citation"International Journal of Molecular Sciences", 2021, Vol. 22, nr 21, e12005pl
dc.identifier.doi10.3390/ijms222112005
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/20.500.12153/2819
dc.language.isoenpl
dc.publisherMDPIpl
dc.relation2016/21/B/ NZ9/01865pl
dc.rightsAttribution 4.0 International (CC-BY 4.0)*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectmidgut microbiotapl
dc.subjectbacterial interactionspl
dc.subjectentomopathogenic nematodespl
dc.subjectXenorhabduspl
dc.subjectPhotorhabduspl
dc.subjectAmphimallon solstitialepl
dc.subjectmikroflora jelitowapl
dc.subjectinterakcje między bakteriamipl
dc.subjectnicienie entomopatogenicznepl
dc.titleScreening and molecular identification of bacteria from the midgut of Amphimallon solstitiale larvae exhibiting antagonistic activity against bacterial symbionts of entomopathogenic nematodespl
dc.typeinfo:eu-repo/semantics/articlepl
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