Intestinal MMC-related electric fields and pancreatic juice control the adhesion of Gram-positive and Gram-negative bacteria to the gut epithelium - in vitro study

Simple item page
dc.contributor.authorJankowska, Alicja
dc.contributor.authorWrzesiński, Michał
dc.contributor.authorLaubitz, Daniel
dc.contributor.authorKazimierczak, Waldemar
dc.contributor.authorSkrzypek, Henryk
dc.contributor.authorBardowski, Jacek
dc.contributor.authorZabielski, Romuald
dc.contributor.authorGrzesiuk, Elżbieta
dc.date.accessioned2022-03-23T08:31:41Z
dc.date.available2022-03-23T08:31:41Z
dc.date.issued2008
dc.description.abstractThe adhesion of six different Lactobacillus and Lactococcus and three pathogenic Escherichia and Salmonella strains was studied using Caco-2 cell line. In this in vitro model system the influence of weak electric field (EF) on bacterial adhesion was tested. The EF source was the in vitro reconstruction of spiking potentials recorded in the duodenum of a healthy calf during one myoelectrical migration complex (MMC) cycle. The ability to adhere to Caco-2 cells of bacteria belonging to two groups, Gram-positive lactobacilli and lactococci, and Gram-negative Escherichia and Salmonella differed considerably. The pathogenic bacteria adhered better to well-differentiated Caco-2 cells whereas lactobacilli and lactococci displayed better adhesion to non-differentiated Caco-2 cells. In the presence of MMC-related EF an increased adhesion of Lactobacillus and Lactococcus but not of Salmonella enterica s. Enteritidis and E. coli 269 to Caco-2 cells was observed. Two later strains adhered even less in the presence of EF. The same tendency was found in the presence of pancreatic juice in a cell medium. In conclusion, the myoelectric component of the small intestinal motility, the MMC-related EF, and pancreatic juice may increase the ability of lactic acid bacteria to adhere to GI epithelial cells, creating better environmental conditions for colonization of the intestine and competition with Gram-negative pathogens.pl
dc.identifier.citation"Journal of Physiology and Pharmacology", 2008, Vol. 59, nr 4, s. 795-810pl
dc.identifier.issn0867-5910
dc.identifier.urihttp://hdl.handle.net/20.500.12153/2851
dc.language.isoenpl
dc.relationPBZ-KBN-093/P06/2003pl
dc.subjectadhesionpl
dc.subjectCaco-2pl
dc.subjectbacteriapl
dc.subjectMMC-related electric fieldpl
dc.titleIntestinal MMC-related electric fields and pancreatic juice control the adhesion of Gram-positive and Gram-negative bacteria to the gut epithelium - in vitro studypl
dc.typeinfo:eu-repo/semantics/articlepl
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Jankowska_Wrzesinski_Laubitz_Kazimierczak_Skrzypek_Bardowski_Zabielski_Grzesiuk_Intestinal_MMC-related_electric_fields.pdf
Size:
509.6 KB
Format:
Adobe Portable Document Format
Description:
Download

License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.63 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections
Artykuły naukowe (WM)