Wydział Medyczny / Faculty of Medicine
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Browsing Wydział Medyczny / Faculty of Medicine by browse.metadata.rights "Uznanie autorstwa 4.0 Międzynarodowe"
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- ItemAddenum to the description of Steinernema jollieti Spiridonov, Krasomil-Osterfeld & Moens, 2004(The Russian Society of Nematologists, 2012-11-14) Skrzypek, Henryk; Kazimierczak, Waldemar; Kreft, Anna; Spiridonov, Sergei; Mráček, ZdeněkAdditonal morphological data are provided for Steinernema jollieti Spiridonov, KrasomilOsterfeld, Moens, 2004. A light and scanning electron microscopy were used to particularize taxonomically important characters missing in the first decsription. The GS% and SW% indices are provided for males. The distribution of genital papillae on male tail is illustrated with SEM images. Structure of female tail and vulvar area are decsribed for females of the first and second generation. The relationships of S. jollieti with other steinernematid feltiae/kraussei group species are discussed.
- ItemCharakterystyka nicieni wyizolowanych z pędraków chrabąszcza Melolontha sp. (Coleoptera: Scarabaeidae)(Instytut Ochrony Roślin - Państwowy Instytut Badawczy, Polskie Towarzystwo Ochrony Roślin, 2012-06-15) Kreft, Anna; Kazimierczak, Waldemar; Sierpińska, Alicja; Skrzypek, Henryk; Skrabucha, Anna; Dybała, Magdalena; Dźwierzyńska, MonikaCelem badań była charakterystyka nicieni porażających pędraki chrabąszcza Melolontha sp. w warunkach naturalnych. Pędraki chrabąszcza były zebrane na terenie nadleśnictw Krasnystaw, Kozienice, Opoczno i Brzeziny, na powierzchniach różniących się warunkami świetlnymi, wilgotnościowymi i temperaturowymi. Identyfikację gatunków przeprowadzono metodą sekwencjonowania fragmentów DNA (deoxyribonucleic acid) oraz metodami morfologicznymi przy zastosowaniu skaningowego mikroskopu elektronowego i mikroskopu optycznego z kontrastem interferencyjno-różnicowym. Uzyskane wyniki wykazały, że pędraki były porażone przez Diplogasteroides magnus oraz Koerneria sp. i Rhabditis sp. Większość zainfekowanych przez nicienie pędraków została zebrana na powierzchniach zadrzewionych, czyli tam, gdzie powierzchnia gleby była dobrze ocieniona, a wilgotność najwyższa.
- ItemEfektywność porażania larw chrabąszcza majowego (Melolontha melolontha L.) przez entomopatogeniczne nicienie(Instytut Ochrony Roślin - Państwowy Instytut Badawczy, Polskie Towarzystwo Ochrony Roślin, 2011-06-15) Kreft, Anna; Skrzypek, Henryk; Kazimierczak, Waldemar; Lech-Pituch, Ewelina; Skrabucha, AnnaExperiments done and presented below aimed at proving that applied species and Polish strains of entomopathogenic nematodes (EPNs) can be used to reduce the number of Melolontha melolontha. Invasive larvae of Steinernema arenarium Bys, S. arenarium Lub, S. feltiae Rap, Heterorhabditis bacteriophora Sie, H. megidis Pla, H. megidis Lub i H. megidis Pla, H. megidis C3 were used in the experiments as biocontrol agents against the larvae L1, L2 and L3 of M. melolontha. The experiments were done at 20°C, in 25 ml boxes filled with pasteurized (3 h at 70°) and air-dried soil. Pathogen doses were 100 IJ/insect. The result showed that susceptibility of M. melolontha to EPNs depends on the insect's developmental stage. Larvae L1 were the most susceptible. S. arenarium Bys and H. megidis strains were more effective in infecting M. melolontha larvae than S. feltiae Rap and H. bacteriohora Sie.
- ItemFunctional traits of Lactobacillus plantarum from fermented Brassica oleracea var. capitata L. in view of multivariate statistical analysis(Springer, 2018-05-04) Polak-Berecka, Magdalena; Kubik-Komar, Agnieszka; Gustaw, Klaudia; Michalak, Magdalena; Kazimierczak, Waldemar; Waśko, AdamIn the present study, Lactobacillus plantarum strain was isolated and identifid from spontaneous fermentation of Brassica oleracea var. capitata L. We used the Unweighted Pair Group Method with Arithmetic Mean Analysis (UPGMA) and Principal Component Analysis (PCA) to examine the variations in the functional properties of the isolates. Six functional traits were analyzed, i.e., viability at low pH, resistance to lysozyme and to SIF, auto- and coaggregation, and ß-glucosidase activity. The present work is the fist study in which the PCA and UPGMA statistical methods were used together to analyze data obtained from the same microbiological experiments. This provided information about the similarity of the examined isolates in terms of their functional traits. Additionally, the level of the analyzed functional traits within the particular groups of isolates was shown. The presented approach is the basis for choosing isolates that are most closely related to the reference strain isolated from pickled cabbage.
- ItemMicrosporidia Nosema spp. – obligate bee parasites are transmitted by air(Nature Research, 2019-10-07) Sulborska, Aneta; Horecka, Beata; Cebrat, Małgorzata; Kowalczyk, Marek; Skrzypek, Tomasz; Kazimierczak, Waldemar; Trytek, Mariusz; Borsuk, GrzegorzMicrosporidia Nosema are transferred among bees via the faecal-oral route. Nosema spp. spores have been detected on fowers and transferred to hives along with the bee pollen. The aim of the present study was to determine whether Nosema microsporidia are transferred by air in an apiary, in a control area (without the presence of bee colonies), and/or in a laboratory during cage experiments with artifcially infected bees. The novel way of transmission by air was investigated by the volumetric method using a Hirst-type aerobiological sampler located on the ground in the apiary, in the Botanical Garden and on the laboratory foor. Concurrently, the mean rate of Nosema infections in the foragers in the apiary was estimated with the Bürker haemocytometer method. Spore-trapping tapes were imaged by means of light microscopy, nomarski interference contrast microscopy and scanning electron microscopy. the highest concentration of Nosema spores per 1m3 of air (4.65) was recorded in August, while the lowest concentration (2.89) was noted in July. This was confrmed by a Real-Time PCR analysis. the presence of N. apis as well as N. ceranae was detected in each of the tested tapes from the apiary. the average copy number of N. apis was estimated at 14.4 × 104 copies per 1 cm2 of the tape; whereas the number of N. ceranae was 2.24 × 104 copies per tape per 1 cm2. the results indicate that Nosema microsporidia were transferred by the wind in the apiary, but not in the Botanical Garden and laboratory by air. This was confrmed by genetic analyses. DNA from immobilised biological material was isolated and subjected to a pcR to detect the Nosema species. A fragment of the 16S rRNA gene, characteristic of Nosema apis and N. ceranae, was detected. our research adds knowledge about the transfer of Nosema spp. microsporidia in the natural environment and indicates the season associated with the greatest risk of a bee colony infection with Nosema spp.
- ItemSteinernema poinari (Nematoda: Steinernematidae): a new symbiotic host of entomopathogenic bacteria Xenorhabdus bovienii(Springer, 2018-06-19) Sajnaga, Ewa; Kazimierczak, Waldemar; Skowronek, Marcin; Lis, Magdalena; Skrzypek, Tomasz; Waśko, AdamThree strains of symbiotic bacteria were isolated from an entomopathogenic nematode Steinernema poinari retrieved from soil in eastern Poland. Using 16S rDNA, recA, gltX, gyrB, and dnaN gene sequences for phylogenetic analysis, these strains were shown to belong to the species Xenorhabdus bovienii. The nucleotide identity between the studied S. poinari microsymbionts and other X. bovienii strains calculated for 16S rDNA and concatenated sequences of four protein-coding genes was 98.7–100% and 97.9–99.5%, respectively. The phenotypic properties of the isolates also supported their close phylogenetic relationship with X. bovienii. All three tested X. bovienii strains of diffrent Steinernema clade origin supported the recovery of infective juveniles and subsequent development of the nematode population. However, the colonization degree of new infective juvenile generations was signifiantly affcted by the bacterial host donor/recipient. The colonization degree of infective juveniles reared on bacterial symbionts deriving from a non-cognate clade of nematodes was extremely low, but proved the possible host-switching between non-related Steinernema species.
- ItemThe contribution of vacuolated foetal-type enterocytes in the process of maturation of the small intestine in piglets(Kielanowski Institute of Animal Physiology and Nutrition, 2018-08-27) Skrzypek, Tomasz; Szymańczyk, Sylwia; Ferenc, Karolina; Kazimierczak, Waldemar; Szczepaniak, Klaudiusz; Zabielski, RomualdIn neonates the vacuolated foetal-type enterocytes (VFE) play a key role in the transport of intact colostral and milk proteins from the lumen of the small intestine into the circulation and/or in the intracellular digestion of intestinal nutrients. The absorption of intact colostral macromolecules (including immunoglobulins, hormones and bioactive peptides) is important in the development of the immune and digestive systems of newborn piglets. The digestion of the intestinal content inside the VFE supports the luminal digestion of nutrients. The presence of apical canalicular system, which produces both the transport and the digestive vacuoles, is a key feature of VFE. The VFEs are gradually replaced by adult-type enterocytes. VFEs disappear gradually from the proximal part of the small intestine to the ileum. VFEs containing large (also referred to as giant) transport vacuoles disappear within the first 2–3 days after birth. VFEs containing digestive vacuoles are present for up to week 3 of life. In contrast, VFEs of intrauterine growth retarded piglets show abnormalities in their development of the apical area. The loss of VFEs is a good marker of the small intestine epithelium maturation.