Browsing by Author "Juda, Marek"

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    Production of enriched in B vitamins biomass of Yarrowia lipolytica grown in biofuel waste
    (Elsevier, King Saud University, 2021) Jach, Monika Elżbieta; Sajnaga, Ewa; Janeczko, Monika; Juda, Marek; Kochanowicz, Elżbieta; Baj, Tomasz; Malm, Anna
    Yarrowia lipolytica as an oleaginous yeast is capable of growing in various non-conventional hydrophobic substrate types, especially industrial wastes. In this study, the content of thiamine (vitamin B1), riboflavin (vitamin B2), pyridoxine (vitamin B6), biotin (vitamin B7) and folic acid (vitamin B9) in the wet biomass of Y. lipolytica strains cultivated in biofuel waste (SK medium), compared to the standard laboratory YPD medium, was assessed. Additionally, the biomass of Y. lipolytica A-101 grown in biofuel waste (SK medium) was dried and examined for B vitamins concentration according to the recommended microbial methods by AOAC Official Methods. The mean values of these vitamins per 100 g of dry weight of Y. lipolytica grown in biofuel waste (SK medium) were as follows: thiamine 1.3 mg/100 g, riboflavin 5.3 mg/100 g, pyridoxine 4.9 mg/100 g, biotin 20.0 µg/100 g, and folic acid 249 µg/100 g. We have demonstrated that the dried biomass is a good source of B vitamins which can be used as nutraceuticals to supplement human diet, especially for people at risk of B vitamin deficiencies in developed countries. Moreover, the biodegradation of biofuel waste by Y. lipolytica is desired for environmental protection.
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    Statistical evaluation of growth parameters in biofuel waste as a culture medium for improved production of single cell protein and amino acids by Yarrowia lipolytica
    (Springer, 2020) Jach, Monika Elżbieta; Baj, Tomasz; Juda, Marek; Świder, Robert; Mickowska, Barbara; Malm, Anna
    Yarrowia lipolytica is an oleaginous yeast species with the ability to grow on a number of substrates types, especially industrial wastes. This paper concerns the statistical optimization of fermentation parameters and media to ensure consistent and improved Y. lipolytica protein production. A strain of Y. lipolytica A-101 was observed to be proficient in producing single cell protein, amino acids, and vitamin B12 while utilizing biofuel waste instead of a complete YPD medium for yeast growth. A fractional fractal design experiment was then applied, and the two fermentation param- eters of temperature and pH were recognized to have a significant effect on the protein and amino acid production. Subsequently, the response surface methodology with a three-level complete factorial design was employed to optimize these influential parameters. Therefore, five different measuring systems were utilized to construct a quad- ratic model and a second-order polynomial equation. Optimal levels of parameters were then obtained by analysis of the model and the numerical optimization method. When the Y. lipolytica A-101 was cultivated at optimized pH (5.0) using biofuel waste as a medium, the protein concentration was increased to 8.28—a 44% enhancement as com- pared to the original (3.65). This study has thus demonstrated a beneficial way to cultivate Y. lipolytica A-101 on biofuel waste for enhanced production of single cell protein and amino acids for use in human diet and in animal feed.
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    Vitamin B12-Enriched Yarrowia lipolytica Biomass Obtained from Biofuel Waste
    (Springer, 2020) Jach, Monika Elżbieta; Masłyk, Maciej; Juda, Marek; Sajnaga, Ewa; Malm, Anna
    The aim of the study was to obtain vitamin B12-enriched biomass of non-conventional yeast Yarrowia lipolytica A-101. The cultivations were performed on waste from biofuel production in aerobic conditions at different temperatures (20–30 °C) and pH values (4.0–7.0) of the medium, with and without the addition of cobalt, L-methionine, molybdenum, δ-aminolevulinic acid, and niacin. A temperature of 30 °C and pH between 5 and 6 were the optimal conditions for obtaining B12-enriched yeast biomass on biofuel waste. In such conditions, Y. lipolytica A-101 contained 9 µg of vitamin B12 per 100 g of dried biomass. The addition of the ingredients mentioned above to the medium did not exert a significant effect on the B12 concentration. Y. lipolytica are able to accumulate this vitamin from the medium in a similar manner to animal cells. Additionally, it should be noted that the dried biomass of Y. lipolytica appeared to be safe for consumption. Taken together, these data showed that Y. lipolytica can be used as a nutritional supplement to increase the intake of vitamin B12, especially at risk of vitamin B12 deficiency.