Masłyk, MaciejJaneczko, MonikaMartyna, AleksandraCzernik, SławomirTokarska-Rodak, MałgorzataChwedczuk, MartaFoll-Josselin, BéatriceRuchaud, SandrineBach, StéphaneDemchuk, Oleg M.Kubiński, Konrad2022-06-222022-06-222019"Molecules", 2019, Vol. 24, nr 1, 153http://hdl.handle.net/20.500.12153/3224Small molecules containing quinone and/or oxime moieties have been found as promising anti-fungal agents. One of them is 4-AN, a recently reported potent anti-Candida compound, which inhibits the formation of hyphae, decreases the level of cellular phosphoproteome, and finally shows no toxicity towards human erythrocytes and zebrafish embryos. Here, further research on 4-AN is presented. The results revealed that the compound: (i) Kills Candida clinical isolates, including these with developed antibiotic resistance, (ii) affects mature biofilm, and (iii) moderately disrupts membrane permeability. Atomic force microscopy studies revealed a slight influence of 4-AN on the cell surface architecture. 4-AN was also shown to inhibit multiple various protein kinases, a characteristic shared by most of the ATP-competitive inhibitors. The presented compound can be used in novel strategies in the fight against candidiasis, and reversible protein phosphorylation should be taken into consideration as a target in designing these strategies.enUznanie autorstwa-Użycie niekomercyjne-Bez utworów zależnych 3.0 Polskahttp://creativecommons.org/licenses/by-nc-nd/3.0/pl/Candida albicansarylcyanomethylenequinones4-ANprotein kinasesantifungal agentatomic force microscopyinfo:eu-repo/semantics/article10.3390/molecules24010153